Manganese inhibits spontaneous nuclear maturation in bovine cumulus-enclosed oocytes
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چکیده
منابع مشابه
DNA Double-Strand Breaks Induce the Nuclear Actin Filaments Formation in Cumulus-Enclosed Oocytes but Not in Denuded Oocytes
As a gamete, oocyte needs to maintain its genomic integrity and passes this haploid genome to the next generation. However, fully-grown mouse oocyte cannot respond to DNA double-strand breaks (DSBs) effectively and it is also unable to repair them before the meiosis resumption. To compensate for this disadvantage and control the DNA repair events, oocyte needs the cooperation with its surroundi...
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Although offspring have been produced from porcine oocytes vitrified at the germinal vesicle (GV) stage, the rate of embryo development remains low. In the present study, nuclear morphology and progression, cumulus expansion, transzonal projections (TZPs), ATP and glutathione (GSH) levels were compared between vitrified cumulus-oocyte complexes (COCs) and control COCs (no cryoprotectant treatme...
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Cumulus cell (CC) apoptosis is inversely correlated with embryonic development in vitro. Therefore, inhibition of CC apoptosis is important for proper embryonic development and quality. Retinoic acids (all-transRA and 9-cisRA) are natural components of retinoids, and 9-cisRA is the physiologically active metabolite of retinoic acid in vitro. During in vitro maturation, 9-cisRA enhances oocyte c...
متن کاملAbsence of cumulus cells during in vitro maturation affects lipid metabolism in bovine oocytes.
Cumulus cells (CC) surround the oocyte and are coupled metabolically through regulation of nutrient intake. CC removal before in vitro maturation (IVM) decreases bovine oocyte developmental competence without affecting nuclear meiotic maturation. The objective was to investigate the influence of CC on oocyte cytoplasmic maturation in relation to energy metabolism. IVM with either cumulus-enclos...
متن کاملEffect of Cumulus Oocyte Complex Morphology on in Vitro Maturation of Bovine Oocytes*
An experimental design was framed to analyse the effect of cumulus oocyte complex (COC) morphology on in vitro maturation of bovine oocytes. Slaughter house derived bovine ovaries from South Indian breeds and crossbred cattle of Kerala were subjected to three retrieval methods to yield different quality grades of oocytes. Based on the number of layers of cumulus cells and ooplasm character, the...
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ژورنال
عنوان ژورنال: Canadian Journal of Animal Science
سال: 2001
ISSN: 0008-3984,1918-1825
DOI: 10.4141/a00-074